Sterol 14-demetilaza

Sterol 14-demetilaza
Identifikatori
EC broj1.14.13.70
CAS broj60063-87-8
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Sterol 14-demetilaza (EC 1.14.13.70, obtusufoliolna 14-demetilaza, lanosterolna 14-demetilaza, lanosterolna 14alfa-demetilaza, sterolna 14alfa-demetilaza) je enzim sa sistematskim imenom sterol,NADPH:kiseonik oksidoreduktaza (14-metil odvajanje).[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju

obtusifoliol + 3 O2 + 3 NADPH + 3 H+ ⇌ {\displaystyle \rightleftharpoons } 4alfa-metil-5alfa-ergosta-8,14,24(28)-trien-3beta-ol + format + 3 NADP+ + 4H2O

Ovaj hem-tiolatni enzim (P-450) katalizuje sukcesivne hidroksilacije 14alfa-metil grupe i C-15, čemu sledi eliminacija formata i formiranje 14(15) dvostruke veze.

Reference

  1. ^ Bak, S., Kahn, R.A., Olsen, C.E. and Halkier, B.A. (1997). „Cloning and expression in Escherichia coli of the obtusifoliol 14α-demethylase of Sorghum bicolor (L.) Moench, a cytochrome P450 orthologous to the sterol 14α-demethylases (CYP51) from fungi and mammals”. Plant J. 11: 191—201. PMID 9076987. CS1 одржавање: Вишеструка имена: списак аутора (веза)
  2. ^ Aoyama, Y. & Yoshida, Y. (1991). „Different substrate specificities of lanosterol 14α-demethylase (P450-14DM) of Saccharomyces cerevisiae and rat liver of 24-methylene-24,25-dihydrolanosterol and 24,25-dihydrolanosterol”. Biochem. Biophys. Res. Commun. 178: 1064—1071. PMID 1872829. 
  3. ^ Aoyama, Y. & Yoshida, Y. (1992). „The 4β-methyl group of substrate does not affect the activity of lanosterol 14α-demethylase (P45014DM) of yeast: differences between the substrate recognition by yeast and plant sterol 14α-demethylases”. Biochem. Biophys. Res. Commun. 183: 1266—1272. PMID 1567403. 
  4. ^ Alexander, K., Akhtar, M., Boar, R.B., McGhie, J.F. and Barton, D.H.R. (1972). „The removal of the 32-carbon atom as formic acid in cholesterol biosynthesis”. J. Chem. Soc. Chem. Commun.: 383—385. CS1 одржавање: Вишеструка имена: списак аутора (веза)

Literatura

  • Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X. 
  • Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036. 
  • Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0. 
  • Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097. 

Spoljašnje veze

  • Sterol+14-demethylase на US National Library of Medicine Medical Subject Headings (MeSH)
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  • B enzm: 1.1/2/3/4/5/6/7/8/10/11/13/14/15-18, 2.1/2/3/4/5/6/7/8, 2.7.10, 2.7.11-12, 3.1/2/3/4/5/6/7, 3.1.3.48, 3.4.21/22/23/24, 4.1/2/3/4/5/6, 5.1/2/3/4/99, 6.1-3/4/5-6
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