PNP agar
PNP Agar is an agar medium used in microbiology to identify Staphylococcus species that have phosphatase activity. The medium changes color when p-nitrophenylphosphate disodium (PNP) is dephosphorylated.[1]
PNP agar is composed of Mueller–Hinton agar buffered to pH 5.6 to 5.8, with the addition of 0.495 mg/mL PNP.[2]
References
- ^ Janda, W.M.; K. Ristow; D. Novak (September 1994). "Evaluation of RapiDEC Staph for Identification of Staphylococcus aureus, Staphylococcus epidermidis, and Staphylococcus saprophyticus". Journal of Clinical Microbiology. 32 (9): 2056–2059. doi:10.1128/jcm.32.9.2056-2059.1994. PMC 263941. PMID 7814525.
- ^ Geary, C.; M. Stevens (October 1989). "Detection of phosphatase production by Staphylococcus species: a new method". Med Lab Sci. 46 (4): 291–294. PMID 2559282.
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Growth media / agar plates
Gram positive |
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Gram negative |
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- BAF agar
- Czapek medium
- Dermatophyte test medium
- Malt extract agar
- MMN medium
- Potato dextrose agar
- Sabouraud agar
- YEPD
- YM
- Blood agar
- Chocolate agar
- Columbia blood agar
- Letheen broth
- Lysogeny broth
- Nutrient agar
- Plate count agar
- Trypticase soy agar
- Tryptic soy broth
- Zobell's marine agar
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